FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12168469
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC7516306.176865799633462No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT6900795.671042100694837No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC6385035.247192559721359No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6154425.057678168058775No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT5577284.583386784319375No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG5345464.3928780194123025No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA3211492.639189860285628No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG1473091.210579572500041No Hit
AACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGA171800.14118456479611363No Hit
TACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGA134730.11072058448766232No Hit
AAAAAAATAGATTTATTAAAGTACAAAACACCAAAAAAAGCAACAATATC127240.10456533192466529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG786650.0142.387191
ATCGATT687450.0142.143881
TCGATTC690750.0141.462452
CGATTCC693600.0140.849333
GATTCCT700550.0139.359154
GATAACG745200.0139.020265
ACTGTAT635850.0138.853853
ATTCCTT705200.0138.522225
AACAATG793200.0138.043292
CGATAGG818350.0137.703926
CTGTATC642100.0137.682394
ATACAGG862550.0137.584037
CGATACA863150.0137.481415
ACGCATT757450.0137.409689
AACGCAT757750.0137.402948
CGCATCT383450.0137.37438
TCGCATC383300.0137.296147
GATACAG864250.0137.256266
TAACGCA758300.0137.227077
GCATCTT384100.0137.160649