FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD8_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD8_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4853571
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC446229091.93828626386635No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC535721.1037646302073256No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCC160560.33080797623028485No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCC119900.24703460606633754No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCA106200.2188079663406593No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCC92500.19058132661498103No Hit
GATCGATAACGCATTTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCC88190.18170126696405595No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCA62760.12930685468493197No Hit
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCC57000.11743930396815046No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCC55180.11368948759583408No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAACGCCAGTAGGTCC54430.11214423359625315No Hit
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCC54170.11160854554306511No Hit
TATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC51810.10674614629105045No Hit
AATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC50150.10332598410531134No Hit
GATCGATAACGCATTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCC49090.10114202511923695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC4820950.0144.918174
GATCGAT4812550.0144.910021
ATAACGC4823650.0144.886676
ATCGATA4824700.0144.868672
GATAACG4818650.0144.862415
TCGATAA4826900.0144.792133
AACGCAT4828600.0144.704158
TAACGCA4829400.0144.668187
ACGCATT4828850.0144.663659
CGTCTGA4642050.0134.35355145
GTCTGAA351650.0118.904526145
CGTCTAA18250.094.13893145
CGTCTGT16200.087.7051145
CGTCAGA19700.087.209915145
CGACTGA16550.085.4123145
CTCTGAA14100.083.8016145
TCTGAAC58950.077.717186145
CGTCGAA13750.076.445145
CGTTGAA13550.072.758446145
ATAACTC9550.070.632576
Produced by FastQC (version 0.11.5)