FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD8_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD8_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7604024
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG544795771.64571021869473No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG161523621.241858258206445No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACGAGGTCCACTATG241190.31718732081855605No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG174360.22929964450401524No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTATG166660.21917342712227103No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG117600.15465495637572949No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG114100.15005213029311848No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATGA96960.12751143341998922No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATGA86110.11324267256389513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTC7554250.0144.896765
CAATGGT7547300.0144.875474
AACAATG7556700.0144.859392
ACAATGG7545450.0144.856223
TAACAAT7555350.0144.845671
ATGGTCT7562250.0144.79916
GGTCTTG7516200.0144.77058
TGGTCTT7563000.0144.689387
GTCTTGT7534900.0144.407369
AACTCCA7292900.0133.16711145
ACTCCAG601950.0122.17289145
AACTCCC33300.0101.22596145
GGTCTTT48650.099.9822548
AACTCAG44050.079.48486145
CAATGAT16350.077.626864
AACTCAA27100.073.56083145
GTCTTTT62050.073.250229
CTCCAGT103350.072.876625145
AACCCAG25900.070.251755145
CAATAGT13150.068.389234