Basic Statistics
Measure | Value |
---|---|
Filename | HH7JLAFX2_n01_0909_CD8_CFSE_low.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7488876 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG | 5355888 | 71.51791537207987 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG | 1591296 | 21.248796214545415 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACGAGGTCCACTATG | 23773 | 0.31744416652111745 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTATG | 18333 | 0.24480309194597427 | No Hit |
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG | 16633 | 0.22210275614124203 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG | 11453 | 0.152933497630352 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG | 10404 | 0.13892605512496134 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATGA | 9177 | 0.12254175392942812 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATGA | 8030 | 0.1072257038305882 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AATGGTC | 744010 | 0.0 | 144.92047 | 5 |
CAATGGT | 743280 | 0.0 | 144.91158 | 4 |
ACAATGG | 743200 | 0.0 | 144.89691 | 3 |
AACAATG | 744370 | 0.0 | 144.89229 | 2 |
TAACAAT | 744280 | 0.0 | 144.88715 | 1 |
ATGGTCT | 744770 | 0.0 | 144.8096 | 6 |
GGTCTTG | 740585 | 0.0 | 144.75237 | 8 |
TGGTCTT | 745010 | 0.0 | 144.67296 | 7 |
GTCTTGT | 742360 | 0.0 | 144.44336 | 9 |
CTGAACA | 18265 | 0.0 | 133.23187 | 145 |
CTGAACT | 701550 | 0.0 | 129.2612 | 145 |
CGTATGC | 2955 | 0.0 | 113.088875 | 145 |
TGAACTC | 76805 | 0.0 | 105.69787 | 145 |
CTGAACC | 2170 | 0.0 | 92.19891 | 145 |
GGTCTTT | 4830 | 0.0 | 90.499596 | 8 |
CTAACTC | 2175 | 0.0 | 90.32053 | 145 |
CGAACTC | 1915 | 0.0 | 85.92777 | 145 |
CAGAACA | 2105 | 0.0 | 77.82745 | 145 |
CAGAACT | 3305 | 0.0 | 75.67007 | 145 |
CAATGAT | 1515 | 0.0 | 74.20255 | 4 |