FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD8_CFSE_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD8_CFSE_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7488876
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG535588871.51791537207987No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG159129621.248796214545415No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACGAGGTCCACTATG237730.31744416652111745No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTATG183330.24480309194597427No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG166330.22210275614124203No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG114530.152933497630352No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG104040.13892605512496134No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATGA91770.12254175392942812No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATGA80300.1072257038305882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTC7440100.0144.920475
CAATGGT7432800.0144.911584
ACAATGG7432000.0144.896913
AACAATG7443700.0144.892292
TAACAAT7442800.0144.887151
ATGGTCT7447700.0144.80966
GGTCTTG7405850.0144.752378
TGGTCTT7450100.0144.672967
GTCTTGT7423600.0144.443369
CTGAACA182650.0133.23187145
CTGAACT7015500.0129.2612145
CGTATGC29550.0113.088875145
TGAACTC768050.0105.69787145
CTGAACC21700.092.19891145
GGTCTTT48300.090.4995968
CTAACTC21750.090.32053145
CGAACTC19150.085.92777145
CAGAACA21050.077.82745145
CAGAACT33050.075.67007145
CAATGAT15150.074.202554