FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD8_CFSE_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD8_CFSE_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6921229
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT644744693.15464059923461No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACGAGGTCCACTAT222770.32186480175702903No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT188410.27222043946241337No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTAT160850.23240092185939806No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT127980.18490935641632433No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT105060.15179385048522454No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT102840.14858632765943738No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT101620.14682363493535613No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG99210.14334159439024485No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT93800.13552506354001578No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT82690.11947300111006297No Hit
ATACTGTATCTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT79090.11427161274392163No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTAT76320.11026943336219623No Hit
ATACTGTATCTCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTAT70470.10181717726721656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT6871650.0145.019551
CTGTATC6879400.0144.919714
ACTGTAT6880850.0144.874423
TACTGTA6883000.0144.829162
GTATCTC6889500.0144.8026
TGTATCT6891600.0144.783135
ATCTCTT6894400.0144.773128
TATCTCT6902150.0144.716637
TCTCTTG6878000.0144.664059
GTCTGAA6601600.0131.38199145
TCTGAAC617700.0115.3649145
GACTGAA29450.0102.893684145
AGGAATG23650.097.78163145
GTCAGAA40800.076.40223145
GTCGAAC20150.075.551315145
ATATCTC14750.075.2333766
GTCTAAC26900.070.60684145
CTATATC15850.070.012144
ACTATAT16800.066.0531163
GCTGAAC15650.064.85022145
Produced by FastQC (version 0.11.5)