FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD8_CD_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD8_CD_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815745
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC624361391.60573055476694No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC763481.1201710157877092No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCC225600.3309982987919883No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCC194820.2858381585578686No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCA144920.21262532562471162No Hit
GATCGATAACGCATTTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCC129550.19007459932846665No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCC119890.17590153387487356No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCA88090.12924485877919434No Hit
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCC85510.1254595058940732No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCC85030.12475525419451579No Hit
TATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC80870.11865173946501813No Hit
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCC78750.11554129445863952No Hit
AATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC77270.11336985171833747No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAACGCCAGTAGGTCC76430.11213741124411199No Hit
GATCGATAACGCATTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCC74960.10998064041421737No Hit
GATCGATAACGCATTTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCC73870.10838140217980573No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGCCAGTAGGTCCA70460.10337828073086655No Hit
GATCGATAACGCATTTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCC69220.1015589638403432No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACAGCCAGTAGGTCC68220.10009177279959858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC6768650.0144.93644
ATAACGC6775450.0144.859446
GATAACG6767300.0144.838855
TCGATAA6779550.0144.738683
AACGCAT6779950.0144.723058
ATCGATA6781700.0144.706682
ACGCATT6781300.0144.683559
TAACGCA6783450.0144.65487
GAGCACA6483450.0130.29552145
GAGCACC84100.0126.709175145
AGCACAC665400.0106.514694145
TTACCTA29800.085.62747145
GAGCTCA16700.074.661835145
GATCGAT13367550.073.236331
AATAACG16100.072.0707865
TCACCCT78650.070.325455145
ATAACTC13400.068.1916056
GACACAC14900.067.62624145
GAGAACA23600.067.57677145
TATCGAT20300.067.183231
Produced by FastQC (version 0.11.5)