FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD8_CD_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD8_CD_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9292079
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC866587293.26085152741383No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTC252720.27197358093920637No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC178850.19247576349706025No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC139400.1500202484287962No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC131390.14140000316398516No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC129370.13922610860282184No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC119370.128464254339637No Hit
CAATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC119090.12816292242026783No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC116830.12573074335678808No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC115600.12440703528241634No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC110770.11920905967329808No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC106790.11492584167655054No Hit
CTATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC97950.10541236250789517No Hit
CGCTCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC93200.10030048173288238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA9198200.0144.943081
GATCGAT9206800.0144.94212
ATCGATA9227800.0144.902283
CGATACA9236700.0144.894585
TCGATAC9237300.0144.891464
ATACAGG9231750.0144.816547
TACAGGT9232750.0144.79388
ACAGGTA9231050.0144.7719
GATACAG9247350.0144.73876
CGTCTGA8848950.0133.55922145
GTCTGAA730450.0113.03632145
CGTCTGT52700.0112.2443145
CGACTGA40900.0100.31778145
GCTCGAT19800.092.312582
CGCTCGA21600.084.646751
CGTCTAA31600.083.50242145
CTCTGAA26300.081.311325145
CGTTGAA26000.078.624985145
GATTGAT19300.078.544272
CAATCGA25200.078.024731
Produced by FastQC (version 0.11.5)