FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7085245
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA660735293.2550956247808No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA233050.3289229941942728No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACAGCCAGTAGGTCCA216060.3049435834611224No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCA193540.27315922032336215No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA130480.1841573580024403No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC107170.1512580016640215No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC93540.13202084049316573No Hit
ATCTATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA75520.10658770444776433No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA71710.10121033217623385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT7041600.0144.932561
CGATTCC7042700.0144.896843
TCGATTC7046250.0144.885572
ATTCCTT7053300.0144.871355
GATTCCT7047450.0144.8644
TTCCTTG7050600.0144.818836
CCTTGGT7043600.0144.740868
TCCTTGG7046950.0144.710117
CTTGGTT7044550.0144.643139
TCTGAAC6677800.0134.39622145
TCTGAAA139850.0129.4331145
CTGAACT482250.0121.41356145
TCAGAAC32950.096.5825145
CTGAACA22450.087.50689145
TCTGACC18750.085.443756145
TGAACTC77500.078.38476145
ACTGAAC19250.075.31623145
CCTTAGT9750.072.8636258
CAATTCC12800.071.9590453
CTATTCC14950.070.827733