FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10272233
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT940824791.58911212391698No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT1002610.9760389975577851No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGT342420.3333452424609138No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT263200.25622471764415783No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGT189240.1842247931876156No Hit
ACGATCGATAGGTAAGGTCTTCTGGAAAGGACGAAACACCGCCAGTAGGT180100.1753270199381186No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGT171210.1666726212304569No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAAACACCGCCAGTAGGT164670.1603059432160466No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGT159050.15483488351559002No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAAACACCGCCAGTAGGT157940.15375430054984152No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGT157290.15312152674106982No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAAACACCGCCAGTAGGT149470.14550877107246302No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAAACACCGCCAGTAGGT145340.14148822364134458No Hit
GCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT144200.14037843573057582No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTC134850.13127622786593723No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT133470.12993280039500663No Hit
ACAATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT117350.1142400099374693No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT106890.10405721910708217No Hit
ATGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT104570.101798703358851No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGCCAGTAGGTC103280.10054289072298106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT10169550.0144.908173
CGATCGA10163250.0144.897382
ATCGATA10184400.0144.896294
CGATAGG10159300.0144.886616
ACGATCG10149000.0144.772721
ATAGGTA10206750.0144.75668
TCGATAG10184600.0144.7565
GATAGGT10166750.0144.749317
TAGGTAA10213400.0144.567259
ACGTCTG9823800.0134.65433145
CGTCTGA700700.0122.88075145
CTATAGG44350.0109.2448046
ACGATCT46550.0108.955961
ACGTCTT55600.0108.86401145
CGATCTA47050.0107.755052
ACGACTG34800.0106.85891145
GATCTAT46400.0106.1377263
ATCTATA49450.099.591314
GCGATCG23500.093.864041
TATAGGT54000.089.403217
Produced by FastQC (version 0.11.5)