FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_CFSE_low_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_CFSE_low_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6872867
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT635519592.46788858274138No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACGAGGTCCACTAT224520.32667589813683284No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT193620.281716494732111No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTAT187650.27303016339469394No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT164980.2400453842624919No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT113920.16575324387915555No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT106500.15495716707452653No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT106310.15468071766847807No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT97960.14253149377108562No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG96150.13989794942925565No Hit
ATACTGTATCTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT88950.1294219719368933No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT88910.12936377206193572No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTAT78770.11461010376019208No Hit
ATACTGTATCTCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTAT74640.10860096667082311No Hit
ATACTATATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT73150.10643302132865368No Hit
ATACTGTATCTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT72560.10557457317302954No Hit
ATACTGTATCTCTTGTGGAAAGCACGAAACACCGCCAGTAGGTCCACTAT71550.10410502633035094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT6820250.0145.008351
ACTGTAT6827850.0144.86743
CTGTATC6829450.0144.864274
TACTGTA6830750.0144.806962
GTATCTC6838650.0144.803016
ATCTCTT6841500.0144.780238
TGTATCT6840250.0144.772315
TATCTCT6849600.0144.72867
TCTCTTG6823250.0144.66079
TGAACTC6530600.0133.9169145
GAACTCC512600.0115.114105145
TGAACAC30850.0100.570656145
TGAACTA48900.097.39558145
TGAACCC19250.079.83399145
TGATCTC16100.079.694824145
AGAACTC24200.073.98851145
TAACTCC21750.072.65742145
ACTATAT15000.070.5931243
CTATATC15150.069.415464
ATATCTC15150.069.415466
Produced by FastQC (version 0.11.5)