FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_CFSE_low_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_CFSE_low_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6328670
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC589070193.07960440345285No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTC180190.28472017027274293No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC123800.19561772062692476No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC95970.15164323625659104No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC91030.14383748876146174No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC88390.13966599617297157No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC82920.1310227899384863No Hit
CGCTCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC82730.13072256888098133No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC81350.12854201593699782No Hit
CAATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC80140.12663008183393984No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC79830.12614024747695804No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC77720.12280621362782385No Hit
CGATCGATACAGGTATTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTC75780.11974079861961517No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC73620.11632775922903232No Hit
CTATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC67450.10657847541426556No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACGCCAGTAGGTCC64550.10199615401024228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA6261950.0144.923891
ATCGATA6283300.0144.899413
CGATACA6292250.0144.885795
TCGATAC6292250.0144.880044
ATACAGG6284850.0144.815787
ACAGGTA6283050.0144.800749
GATCGAT6275150.0144.775542
GATACAG6295200.0144.770666
TACAGGT6287300.0144.745518
CACGTCT5996700.0132.99388145
CACGTCG41150.0120.84662145
CAAGTCT21300.097.674805145
CCGCGCG95450.095.99562145
ATACAGA13800.095.0779347
GCTCGAT17350.094.889762
CACGTTG11900.094.42021145
ACGTCTG497650.094.12894145
CACCTCT23350.092.51446145
CGCTCGA17950.091.7448041
CACGTCA23200.091.23786145
Produced by FastQC (version 0.11.5)