FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_CFSE_high_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_CFSE_high_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA3470.83333333333334No Hit
GATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA612.5No Hit
CATAGGTCGCATCTAGTGGAAAGGAAGAAACACCACCACTACCTCAACTA12.083333333333333No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAACTA12.083333333333333No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGCTCCACTA12.083333333333333No Hit
GATAGGTAAGGTCTTGTGGAAAGGACGAAACCCGCCAGTAGGTCCACTAT12.083333333333333No Hit
GATAGGTAACGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA12.083333333333333No Hit
GATAGGTCGTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGAG12.083333333333333No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTCTTCCGATCTACGA12.083333333333333No Hit
GATAGGTAACGTCTTTAGGAAAGGACGAAACAACGCCAGTAGGTCCACTA12.083333333333333No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)