FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_CFSE_high_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_CFSE_high_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184160
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT382339591.37783927956866No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT412630.9861716569156055No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGT149470.3572282130702459No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT108350.25895281251194985No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGT77320.18479216855951971No Hit
ACGATCGATAGGTAAGGTCTTCTGGAAAGGACGAAACACCGCCAGTAGGT75730.18099212267217313No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGT75350.180083935604757No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAAACACCGCCAGTAGGT69080.16509884899239036No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAAACACCGCCAGTAGGT67410.161107605827693No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGT67020.160175519100608No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGT65300.15606477763756643No Hit
GCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT63720.15228863140988874No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAAACACCGCCAGTAGGT63670.15216913311154448No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAAACACCGCCAGTAGGT63140.15090245114909565No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT56790.13572616725937822No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTC54240.1296317540438224No Hit
ACAATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT50870.12157756873542121No Hit
ATGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT46560.11127681541814845No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT45270.10819375932086728No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGCCAGTAGGTC43450.10384402126113723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4124950.0144.98411
CGATAGG4136500.0144.851886
GATCGAT4141850.0144.841613
ATCGATA4149300.0144.812294
GATAGGT4136350.0144.800137
ATAGGTA4154350.0144.759028
TCGATAG4146600.0144.747425
TAGGTAA4157000.0144.579549
CGATCGA4150450.0144.433152
CACACGT3958850.0132.47058145
CACACGG25700.0126.08238145
CTATAGG18600.0110.344516
AACACGT18100.0109.73672145
ACGATCT19500.0107.900231
ACACGTC333500.0107.355225145
GATCTAT20050.0104.173043
CGATCTA20450.0102.845932
ACACGTG11250.0101.16427145
ATCTATA22550.092.6239244
CACACTC15550.088.1072145
Produced by FastQC (version 0.11.5)