FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_CD_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_CD_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9456472
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA865988691.5762876472325No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA535580.566363438711604No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTA340680.36026120523594846No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTA249410.2637452952855991No Hit
GATAGGTCGCATCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTA189100.1999688678822292No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTA178880.18916145471588136No Hit
GATAGGTCGCATCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTA175650.18574580456643874No Hit
GATAGGTCGCATCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTA168760.17845978923217876No Hit
GATAGGTCGCATCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTA165440.17494896616835537No Hit
GATAGGTCGCATCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTA152490.16125464126579128No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA150610.15926658483206No Hit
GATAGGTCGCATCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTA139080.14707387702305894No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTAT134630.14236810514534384No Hit
GATAAGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA127020.13432070649603786No Hit
GATAGATCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA123220.13030229455551712No Hit
AATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA116450.12314317644043149No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTAT114170.12073212927611904No Hit
GATAGGTCACATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA110790.1171578576027085No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACGCCAGTAGGTCCACTAT94920.10037570036690216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC9376100.0144.92114
GATAGGT9375600.0144.869671
GGTCGCA9378400.0144.860815
CGCATCT9390150.0144.817638
TAGGTCG9381800.0144.812943
GCATCTT9388500.0144.766659
ATAGGTC9399650.0144.739332
TCGCATC9397550.0144.701267
GTCGCAT9388700.0144.665596
ACGTCTG8921550.0131.77528145
ACGACTG51250.0115.842964145
CGTCTGA816800.0112.02797145
ATCGCAT20700.096.3523946
ACGTCTA35250.090.895584145
TCGCAAC28950.089.141737
ACGTCAG62700.088.32943145
AAGTCGC24350.084.5900964
ACGTCGA28150.082.14704145
GATAAGT25300.081.721641
GCAACTT31900.081.579949
Produced by FastQC (version 0.11.5)