FastQCFastQC Report
Thu 17 Sep 2020
HH7JLAFX2_n01_0909_CD4_CD_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7JLAFX2_n01_0909_CD4_CD_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5719835
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA531241692.87708474108082No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA187580.32794652293291676No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACAGCCAGTAGGTCCA172970.3024038280824534No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCA143510.25089884585831584No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA117520.20546047219893582No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC82750.14467200539875713No Hit
ATCGATTCCTTGGTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCA76160.13315069403225793No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA72130.12610503624667493No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC71820.1255630625708609No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCA66920.11699638188863841No Hit
ATCGATTCCTTGGTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCA65810.115055766468788No Hit
ATCTATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA64970.11358719263754985No Hit
ATCGATTCCTTGGTTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCA63160.11042276569166769No Hit
ATCGATTCCTTGGTTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCA60700.10612194232875599No Hit
ATCGATTCCTTGATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA58760.10273023609946791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCC5685600.0144.88583
ATTCCTT5693000.0144.879655
GATTCCT5688050.0144.86424
TCGATTC5689600.0144.862982
ATCGATT5687900.0144.849851
TTCCTTG5688750.0144.818336
CCTTGGT5681600.0144.730538
TCCTTGG5683050.0144.712747
CTTGGTT5683100.0144.617079
GCACACC145300.0137.35104145
GCACACG5336750.0133.53754145
CACACGT422250.0114.32224145
GAACACG20800.091.31232145
GCAGACG9100.086.83134145
CCTTAGT10150.079.991418
CACACGG9600.075.51273145
GACACGT8600.071.64928145
GCACACT15900.071.124435145
CTATTCC14300.070.497123
GCAACGT9100.067.71251145
Produced by FastQC (version 0.11.5)