FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15395944
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9565246.212831119676715No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2470411.6045849478278176Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA713650.066.881832
TGACTGG307800.066.5096742
GTGACTG314050.066.3153461
GATAGTC344650.066.07104510
TAGTCGT215900.065.7331712
ATAGTCG279200.065.18935411
ACTGGAG322550.065.0167854
GGAGTTC259050.064.6933447
ACTGAAG711000.064.260954
ATAACTG752800.063.3611031
CGTGTGC252200.063.3455217
ACGTGTG259200.063.27815216
TGAAGAT800950.062.848026
TGGAGTT332500.062.8440936
AGTTCAG262750.062.692369
TCAGACG252400.062.65929412
CTGAAGA801600.062.6063275
GAGTTCA269900.062.344568
GTGTGCT244350.061.82563818
ATTCGTG111950.061.28852513
Produced by FastQC (version 0.11.5)