FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448808
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1297672.381566757353168Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257660.47287406713541746No Hit
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA85340.15662141150871897No Hit
GAGGTACTTCATGCGAAAGCAGTTGAAGACAAGTTCGAAAAGAGTTTGGA66170.12143940472852044No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC65970.12107235197129354Illumina Single End PCR Primer 1 (98% over 50bp)
GGAGGTACTTCATGCGAAAGCAGTTGAAGACAAGTTCGAAAAGAGTTTGG63820.11712653483110436No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC61000.11195109095420504Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC237000.047.5305649
CGCCGTA266400.047.1307647
TCGCCGT266650.046.77324346
CGTATCA242000.046.7365750
GTATCAT236100.045.53223851
GTGACTG26500.045.079471
GCCGTAT277700.044.87467648
GTCGCCG285400.044.64477545
GGTCGGA15550.044.569542
GGTCGCC289550.044.48847244
CTCGGTG259350.044.17200538
GTGGTCG293400.043.8092542
GATCTCG254450.043.78421435
TCTCGGT258100.043.7214337
ATCTCGG260700.043.3252736
GGTGGTC300550.043.2678541
TGGTCGC296950.043.22657443
GACTGGA32050.042.802553
AGATCTC264800.042.2446934
TATCATT257900.042.23995652