Basic Statistics
Measure | Value |
---|---|
Filename | HH7CGAFX2_n02_ncm10-9.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5448808 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 129767 | 2.381566757353168 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 25766 | 0.47287406713541746 | No Hit |
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA | 8534 | 0.15662141150871897 | No Hit |
GAGGTACTTCATGCGAAAGCAGTTGAAGACAAGTTCGAAAAGAGTTTGGA | 6617 | 0.12143940472852044 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 6597 | 0.12107235197129354 | Illumina Single End PCR Primer 1 (98% over 50bp) |
GGAGGTACTTCATGCGAAAGCAGTTGAAGACAAGTTCGAAAAGAGTTTGG | 6382 | 0.11712653483110436 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 6100 | 0.11195109095420504 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 23700 | 0.0 | 47.53056 | 49 |
CGCCGTA | 26640 | 0.0 | 47.13076 | 47 |
TCGCCGT | 26665 | 0.0 | 46.773243 | 46 |
CGTATCA | 24200 | 0.0 | 46.73657 | 50 |
GTATCAT | 23610 | 0.0 | 45.532238 | 51 |
GTGACTG | 2650 | 0.0 | 45.07947 | 1 |
GCCGTAT | 27770 | 0.0 | 44.874676 | 48 |
GTCGCCG | 28540 | 0.0 | 44.644775 | 45 |
GGTCGGA | 1555 | 0.0 | 44.56954 | 2 |
GGTCGCC | 28955 | 0.0 | 44.488472 | 44 |
CTCGGTG | 25935 | 0.0 | 44.172005 | 38 |
GTGGTCG | 29340 | 0.0 | 43.80925 | 42 |
GATCTCG | 25445 | 0.0 | 43.784214 | 35 |
TCTCGGT | 25810 | 0.0 | 43.72143 | 37 |
ATCTCGG | 26070 | 0.0 | 43.32527 | 36 |
GGTGGTC | 30055 | 0.0 | 43.26785 | 41 |
TGGTCGC | 29695 | 0.0 | 43.226574 | 43 |
GACTGGA | 3205 | 0.0 | 42.80255 | 3 |
AGATCTC | 26480 | 0.0 | 42.24469 | 34 |
TATCATT | 25790 | 0.0 | 42.239956 | 52 |