FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6887846
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4570126.6350496221895785Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG538330.7815650930639274No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC207510.30126980190904384Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC198940.28882759573892913Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC158400.22997029840678782Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC127180.18464408176373281Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC92860.13481718377559546Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC80460.11681445839526611Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG101700.062.656081
TGACTGG103000.061.854772
GACTGGA118050.058.700313
ACTGGAG123200.055.948644
GGTCGGA36300.054.6783562
GATCGAG42550.052.7822048
GAGTTAG81050.050.5855678
CTGGAGT108300.050.4913835
AGTTAGA88400.046.7002949
GTATCAT798850.046.4395751
CCGTATC835700.046.23066749
GTTAGAT89150.046.0327110
CGTATCA848450.045.68034750
AGATAGA118850.045.240437
GATAGAT117700.045.147468
TATCATT833250.045.10629752
AGGTCGG44000.044.903261
CATTAAA867000.044.88903855
CGCCGTA956450.044.76379447
TCGCCGT958200.044.437346
Produced by FastQC (version 0.11.5)