FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350726
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4911206.681244818539013Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564710.7682370421642706No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC211470.28768586939575763Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC210530.2864070841437975Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC175600.23888796834489545Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC142140.19336865501448428Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC102400.1393059678731053Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC86280.11737616121183132Illumina Single End PCR Primer 1 (100% over 49bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA85280.11601575136932053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG107350.062.3281141
TGACTGG108950.060.9866522
GACTGGA127150.057.8018463
GGTCGGA38250.054.365182
ACTGGAG135750.054.0744484
GATCGAG50200.053.9403158
GAGTTAG88600.052.6742488
CTGGAGT118750.049.377765
AGTTAGA99100.047.730299
GTATCAT860400.046.59574551
CCGTATC902450.046.2279649
AGATAGA131100.045.7654467
CGTATCA915600.045.7554950
GTTAGAT102250.045.57554610
GATAGAT130000.045.3453568
AGGTCGG46000.045.3118551
TATCATT895100.045.2430352
CATTAAA923050.045.19719355
ATCATTA914200.044.60793353
CGCCGTA1028650.044.54231647
Produced by FastQC (version 0.11.5)