FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6746336
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3509595.202216432742158Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG576270.8541970041219411No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC174470.25861445383093873Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA118960.1763327530677393No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC84940.12590538034275198Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC81700.12110277341656271Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG80600.060.1984331
TGACTGG81000.059.245082
GACTGGA93600.056.6363073
ACTGGAG97700.053.1720964
CCGTATC622950.052.9301349
GTATCAT615100.052.1608451
CGTATCA641600.051.92626650
CGCCGTA671050.051.54170247
TCGCCGT667100.051.1868946
GATCGAG36050.050.8416868
TATCATT638250.050.2743952
CATTAAA690450.049.65870355
ATCATTA671750.049.32541753
GGTCGGA33300.049.29792
TCATTAA684150.048.83088754
GCCGTAT716250.048.42700248
GTCGCCG754400.048.1356645
TGGTCGC742050.048.12070543
GGTCGCC758300.048.0912144
GATCTCG713450.047.7727635