Basic Statistics
Measure | Value |
---|---|
Filename | HH7CGAFX2_n02_ncm10-6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6746336 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 350959 | 5.202216432742158 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 57627 | 0.8541970041219411 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 17447 | 0.25861445383093873 | Illumina Single End PCR Primer 1 (98% over 50bp) |
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA | 11896 | 0.1763327530677393 | No Hit |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC | 8494 | 0.12590538034275198 | Illumina Single End PCR Primer 1 (100% over 38bp) |
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 8170 | 0.12110277341656271 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTGACTG | 8060 | 0.0 | 60.198433 | 1 |
TGACTGG | 8100 | 0.0 | 59.24508 | 2 |
GACTGGA | 9360 | 0.0 | 56.636307 | 3 |
ACTGGAG | 9770 | 0.0 | 53.172096 | 4 |
CCGTATC | 62295 | 0.0 | 52.93013 | 49 |
GTATCAT | 61510 | 0.0 | 52.16084 | 51 |
CGTATCA | 64160 | 0.0 | 51.926266 | 50 |
CGCCGTA | 67105 | 0.0 | 51.541702 | 47 |
TCGCCGT | 66710 | 0.0 | 51.18689 | 46 |
GATCGAG | 3605 | 0.0 | 50.841686 | 8 |
TATCATT | 63825 | 0.0 | 50.27439 | 52 |
CATTAAA | 69045 | 0.0 | 49.658703 | 55 |
ATCATTA | 67175 | 0.0 | 49.325417 | 53 |
GGTCGGA | 3330 | 0.0 | 49.2979 | 2 |
TCATTAA | 68415 | 0.0 | 48.830887 | 54 |
GCCGTAT | 71625 | 0.0 | 48.427002 | 48 |
GTCGCCG | 75440 | 0.0 | 48.13566 | 45 |
TGGTCGC | 74205 | 0.0 | 48.120705 | 43 |
GGTCGCC | 75830 | 0.0 | 48.09121 | 44 |
GATCTCG | 71345 | 0.0 | 47.77276 | 35 |