FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5755460
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3564356.192988918348838Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG417950.7261800099383889No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC162920.2830703366889875Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC147890.2569560035166607Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC136390.23697497680463422Illumina Single End PCR Primer 1 (100% over 38bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA111940.19449357653428223No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC102440.1779875109895647Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC80540.13993668620753164Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC75270.13078016353167254Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG85350.062.5452271
TGACTGG86650.061.638922
GACTGGA101450.057.670693
GGTCGGA29750.055.529542
ACTGGAG105150.054.661114
GATCGAG34850.051.5899628
GAGTTAG67900.049.5060738
CTGGAGT92350.048.9383545
AGATAGA100650.046.708317
AGTTAGA72600.046.496289
CCGTATC653950.046.490349
GTATCAT627500.046.424951
CGTATCA662750.045.97863450
AGGTCGG35950.045.6968961
GATAGAT102000.045.5410238
CATTAAA676850.045.0009155
CGCCGTA748200.044.90321447
TATCATT655800.044.77914452
TCGCCGT748450.044.7019246
TCATTAA670000.044.405254
Produced by FastQC (version 0.11.5)