HH7CGAFX2_n02_ncm10-4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HH7CGAFX2_n02_ncm10-4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6311057
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	205537	3.25677616285196	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	35954	0.5696985465350732	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	9008	0.14273361815619792	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	7800	0.123592608971841	Illumina Single End PCR Primer 1 (100% over 38bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	7676	0.1216278033933143	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	5315	53.32253	1
TGACTGG	5425	52.269417	2
GACTGGA	5875	52.18037	3
ACTGGAG	6560	46.40238	4
CCGTATC	38795	44.556053	49
GGTCGGA	1860	44.041824	2
CGTATCA	39630	43.776257	50
GTATCAT	38120	43.51797	51
CGCCGTA	43830	43.13269	47
TCGCCGT	43900	42.81708	46
CTGGAGT	5390	42.383244	5
GATCGAG	2270	41.917194	8
GCCGTAT	45265	41.202427	48
CATTAAA	42290	41.139633	55
TATCATT	40900	41.0222	52
CTCGGTG	43350	40.878193	38
ATCATTA	41590	40.812958	53
GTCGCCG	47255	40.688263	45
GATCTCG	42530	40.530342	35
TCTCGGT	43200	40.50971	37