FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358903
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1848944.241755322382719Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316000.7249530443783677No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC87960.20179389172000384Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC45300.10392523072892423Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG43700.058.446671
TGACTGG46200.055.69422
GACTGGA50150.055.2712443
CCGTATC318950.052.94189549
GTATCAT316900.051.88156551
CGCCGTA343900.051.826447
CGTATCA330900.051.61177450
ACTGGAG54700.051.302364
TCGCCGT343500.051.25556646
TATCATT334100.049.32586752
GCCGTAT364550.048.8048348
ATCATTA348800.048.64198353
CATTAAA364150.048.46656855
GTCGCCG389500.048.1318945
TCATTAA356900.048.04858854
GGTCGCC392600.047.97472844
TGGTCGC385500.047.70517343
CTCGGTG379900.047.4734538
GATCTCG372600.047.37029335
ATCTCGG378300.047.29491436
Produced by FastQC (version 0.11.5)