FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429586
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2644584.113141965905736Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG536070.8337550815869016No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC123360.1918630530799339Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA93800.14588808672906778No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC71210.11075363172683281Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG65450.057.2758672
GTGACTG65100.057.1479261
GACTGGA75550.054.8893243
ACTGGAG81700.050.099034
CCGTATC478100.049.8694649
CGTATCA494200.048.71940650
GTATCAT477150.048.68486851
CGCCGTA520650.048.5878547
GGTCGGA25750.048.3910032
TCGCCGT519700.048.11201546
TATCATT498550.046.6793552
CATTAAA544450.045.6516455
GCCGTAT551550.045.6193248
ATCATTA525050.045.53680853
GTCGCCG590150.044.80641645
TCATTAA538800.044.7977354
GGTCGCC595350.044.5914544
TGGTCGC582900.044.5710243
GATCGAG29250.044.4879158
CTCGGTG565900.044.4504538
Produced by FastQC (version 0.11.5)