HH7CGAFX2_n02_ncm10-21.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HH7CGAFX2_n02_ncm10-21.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6278580
Sequences flagged as poor quality	0
Sequence length	76
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	273953	4.363295522235919	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	40066	0.6381379229061348	No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	16583	0.26412023100764825	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	9268	0.14761299529511449	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	7228	0.11512157207521445	Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	6723	0.1070783521114647	Illumina Single End PCR Primer 1 (100% over 40bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	5335	55.87896	1
GGTCGGA	3170	53.884953	2
TGACTGG	5635	52.675526	2
GACTGGA	6330	51.742935	3
ACTGGAG	6880	48.813965	4
CCGTATC	54010	46.510746	49
GATCGAG	3210	46.421688	8
GTATCAT	52040	46.41484	51
CGTATCA	55040	45.89476	50
CGCCGTA	60610	45.128036	47
TCGCCGT	60645	44.767933	46
TATCATT	54590	44.49039	52
CATTAAA	57465	43.909637	55
CTGGAGT	6200	43.838882	5
ATCATTA	56340	43.51854	53
TCATTAA	56690	43.324306	54
GAGTTAG	5465	42.820553	8
GCCGTAT	62935	42.76598	48
AGGTCGG	3995	42.52831	1
CTCGGTG	60555	42.100315	38