HH7CGAFX2_n02_ncm10-20.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HH7CGAFX2_n02_ncm10-20.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5850817
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	218358	3.732094167361584	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	31814	0.5437531202907218	No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	12362	0.21128673140862206	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	8927	0.15257698198388361	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	6992	0.1195046777227864	Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	6115	0.10451531811711083	Illumina Single End PCR Primer 1 (100% over 40bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	5515	56.650017	1
TGACTGG	5545	56.4929	2
GACTGGA	6470	53.75984	3
ACTGGAG	7210	49.252544	4
GATCGAG	2790	47.896236	8
GGTCGGA	2290	47.838905	2
CTGGAGT	6240	45.12993	5
CCGTATC	43815	43.9503	49
GAGTTAG	5470	43.9351	8
GTATCAT	42175	43.692184	51
CGTATCA	44740	43.19028	50
CGCCGTA	49990	42.433628	47
TCGCCGT	50260	41.949055	46
TATCATT	44520	41.854702	52
CATTAAA	46945	41.198925	55
ATCATTA	46015	40.966537	53
TCATTAA	46570	40.561	54
AGTTAGA	6015	40.30599	9
GCCGTAT	52380	39.910748	48
CTCGGTG	50010	39.47317	38