HH7CGAFX2_n02_ncm10-1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HH7CGAFX2_n02_ncm10-1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5524013
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	185621	3.3602563933140637	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	28082	0.5083623083435901	No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	8795	0.1592139627477343	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	8705	0.15758471241830893	Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA	7809	0.1413646202498075	No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	6917	0.12521693920705834	Illumina Single End PCR Primer 1 (100% over 38bp)
GAGGTACTTCATGCGAAAGCAGTTGAAGACAAGTTCGAAAAGAGTTTGGA	6218	0.11256309498185468	No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	6010	0.10879771644273827	Illumina Single End PCR Primer 1 (100% over 40bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	5965	58.251377	1
TGACTGG	6035	57.011497	2
GACTGGA	6800	55.11533	3
ACTGGAG	7465	50.660995	4
GGTCGGA	1755	47.865257	2
CTGGAGT	6385	46.40586	5
GATCGAG	2460	43.93939	8
GTATCAT	37460	41.780186	51
CCGTATC	39480	41.39808	49
CGTATCA	40205	40.86923	50
ATAGTCG	1780	40.68553	11
CTTAGGG	1845	40.604897	61
GAGTTAG	4445	40.29293	8
CGCCGTA	45300	39.84821	47
TATCATT	40135	39.562454	52
TCGCCGT	45360	39.49526	46
CATTAAA	42140	39.301006	55
ATCATTA	41115	39.09624	53
TCATTAA	41495	38.729774	54
GCCGTAT	47735	37.75055	48