FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102188
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4621977.5742831915372Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG493880.8093490400492414No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC225330.36926099294220366Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC211170.34605620148051813Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC155500.2548266293991598Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC124830.20456596879676603Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC79700.13060888979493912Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC62600.10258615434332735Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG95850.062.636311
TGACTGG98050.061.3315352
GACTGGA114250.057.5573123
GGTCGGA37550.056.117052
ACTGGAG119450.054.800674
GAGTTAG83600.053.394488
GATCGAG39650.053.201778
CTGGAGT97550.051.3899275
AGTTAGA89300.050.4972429
AGGTCGG41800.049.7757341
GTTAGAT93950.047.70002410
GTATCAT796950.047.574551
CCGTATC842050.047.02602449
CGTATCA850250.046.6877750
GGAGTTA96550.046.6679957
TATCATT826700.046.44257452
CATTAAA861750.046.1349255
ATCATTA843600.045.99352653
TCATTAA845150.045.93101554
AGATAGA105100.045.6341827
Produced by FastQC (version 0.11.5)