HH7CGAFX2_n02_ncm10-18.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HH7CGAFX2_n02_ncm10-18.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5664050
Sequences flagged as poor quality	0
Sequence length	76
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	379458	6.699411198700576	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	47377	0.8364509494089918	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	19323	0.3411516494381229	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	15241	0.2690830765971346	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	14177	0.25029793169198716	Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	10898	0.19240649358674447	Illumina Single End PCR Primer 1 (100% over 40bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	9170	62.614666	1
TGACTGG	9215	62.07492	2
GACTGGA	10430	58.624077	3
ACTGGAG	10745	56.073788	4
GGTCGGA	2925	53.498432	2
GAGTTAG	7360	52.617954	8
CTGGAGT	8710	51.213333	5
GATCGAG	3200	49.304974	8
AGTTAGA	8085	48.247017	9
GTATCAT	64035	47.705944	51
CCGTATC	67790	47.28856	49
GTTAGAT	8210	46.83065	10
CGTATCA	68755	46.75213	50
TATCATT	66700	46.392883	52
CATTAAA	69840	45.946835	55
ATCATTA	68175	45.907738	53
TCATTAA	68390	45.800053	54
CGCCGTA	78050	45.78384	47
AGGTCGG	3415	45.649475	1
TCGCCGT	78030	45.540283	46