FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7542580
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1603632.1261027393809546Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302120.40055259606129473No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC77900.10328030992047814Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG44100.046.0760881
CCGTATC295000.045.93446449
CGCCGTA323750.044.99910447
TCGCCGT326550.044.63469346
CGTATCA305000.044.54318650
TGACTGG45850.044.207012
GACTGGA50800.043.8852043
GTATCAT300850.043.37738451
GCCGTAT338800.042.6491848
GGTCGCC359500.042.21841444
GTCGCCG355700.042.17740245
CTCGGTG332400.041.6441338
GTGGTCG366700.041.16992642
TCTCGGT330800.041.14724737
ATCTCGG333050.041.04764636
GATCTCG329050.041.02543635
GGTGGTC373900.040.63926741
TGGTCGC370300.040.4955543
TATCATT328750.040.0794152
GGTCGGA19050.039.69272
Produced by FastQC (version 0.11.5)