HH7CGAFX2_n02_ncm10-16.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HH7CGAFX2_n02_ncm10-16.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7301019
Sequences flagged as poor quality	0
Sequence length	76
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	589073	8.068366895086838	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	53662	0.734993293401921	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	24806	0.33976079229488376	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	20749	0.2841932064551537	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	15974	0.2187913769297135	Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	14959	0.20488920793111207	Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	7938	0.10872454927182082	Illumina Single End PCR Primer 1 (100% over 49bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	10415	60.37243	1
TGACTGG	10560	59.699085	2
GACTGGA	11735	57.227215	3
GGTCGGA	3450	54.07867	2
ACTGGAG	12365	53.82039	4
GTATCAT	91675	52.4336	51
CCGTATC	95395	52.388763	49
CGTATCA	96645	51.81983	50
TATCATT	94875	51.174248	52
CGCCGTA	107505	50.951267	47
CATTAAA	98600	50.935425	55
TCGCCGT	107360	50.6165	46
ATCATTA	96800	50.586903	53
TCATTAA	97440	50.283726	54
GCCGTAT	109605	49.48529	48
GAGTTAG	7570	48.982643	8
GATCGAG	4350	48.973404	8
GTCGCCG	113695	48.83985	45
CTCGGTG	103410	48.769413	38
GATCTCG	100865	48.72644	35