FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5894757
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC85079714.433114036761822Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG725471.2307038271467339No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC281260.4771358683657359Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC213120.36154162079963603Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC212100.3598112695739621Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC180700.3065435945875292Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC123020.20869392919843854Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC80970.1373593517086455Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT77730.13186294193297537Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC75060.1273334931363583Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA73920.12539957117825212No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC73510.12470403784244204Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC73240.1242460036944695Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC63920.10843534347556649Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG138800.066.299071
TGACTGG141200.065.519032
GACTGGA157550.062.3077433
ACTGGAG159900.061.0711024
GGTCGGA44500.058.0498772
GAGTTAG93950.057.2315038
CTGGAGT124350.056.437185
GTATCAT1263800.054.98196851
GATCGAG48100.054.9110768
CCGTATC1321850.054.49530849
TATCATT1288500.054.17249752
CGTATCA1339550.054.07837750
CATTAAA1366500.053.63781455
ATCATTA1329400.053.60651453
TCATTAA1339400.053.4602554
CGCCGTA1445700.053.0409247
TCGCCGT1441950.052.77375446
CTGGAGA69100.052.65455
AGTTAGA104200.052.241839
AGGTCGG49850.051.508111
Produced by FastQC (version 0.11.5)