FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241425
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5820699.325899133611316Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG561640.8998586060074423No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC266280.4266333409437748Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC219310.351378090740496Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC164160.26301685913072737Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC145950.2338408296182362Illumina Single End PCR Primer 1 (100% over 40bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA123960.19860849084944546No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC92680.14849173065445792Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC65870.10553679648477711Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC63830.10226831212423444Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG102550.062.9006461
TGACTGG103600.062.6465152
GACTGGA118100.059.6220173
ACTGGAG123750.056.6030164
GGTCGGA38450.056.265092
GATCGAG40550.052.974088
GAGTTAG76600.052.752028
CTGGAGT101450.052.0395475
GTATCAT907550.051.0013951
CCGTATC958500.050.653249
CGTATCA971050.050.14634750
TATCATT940300.049.82067552
CATTAAA978650.049.41076355
AGTTAGA82700.049.329119
CGCCGTA1101400.049.15563647
ATCATTA961950.049.12150653
TCATTAA964650.049.04207254
TCGCCGT1101200.048.92178346
AGGTCGG44850.048.2795261
GCCGTAT1123500.047.6150448
Produced by FastQC (version 0.11.5)