FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944307
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC62803110.565251761054736Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG591380.9948678626457214No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC267130.44938796061508934Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC203660.34261352921375027Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC148530.24986932875438633Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC129810.21837701181988078Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC93990.15811767460866338Illumina Single End PCR Primer 1 (100% over 49bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA93490.15727653366489988No Hit
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC79230.13328719394876476Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC63230.10637068374833264Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG85600.062.723421
TGACTGG85250.062.57642
GACTGGA99150.058.8398443
GGTCGGA42400.058.450392
ACTGGAG103250.056.8966834
GTATCAT994450.051.75476551
GATCGAG38200.051.742298
CTGGAGT82200.051.7106065
AGGTCGG47900.051.6946071
CCGTATC1035150.051.53241349
GAGTTAG67250.051.187438
CGTATCA1047100.051.12482550
TATCATT1024650.050.6188252
CATTAAA1069450.050.41855655
CGCCGTA1146850.050.24977547
ATCATTA1046000.050.1913653
TCATTAA1049600.050.13429354
TCGCCGT1147100.049.9772446
CTCGGTG1132600.048.39893738
GCCGTAT1176900.048.3263548
Produced by FastQC (version 0.11.5)