FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758862
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1619282.395787929979929Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311060.46022540480927115No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC77130.11411684393023559Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC291000.047.7801849
CGCCGTA326550.047.13234747
TCGCCGT330150.046.4706646
CGTATCA299600.046.4670850
GTATCAT294750.045.43836651
GTGACTG36950.045.2207571
GCCGTAT337600.045.05116748
GTCGCCG350050.044.78882645
CTCGGTG312450.044.7873438
GGTCGCC355700.044.6383244
GATCTCG307500.044.4610935
TCTCGGT311350.044.38347237
ATCTCGG314500.044.10587336
GTGGTCG359650.043.9923442
TGACTGG38700.043.5041122
GGTCGGA16050.043.3984532
GGTGGTC368250.043.383241
TGGTCGC366050.043.1084343
TCGGTGG355500.042.85868539
AGATCTC321200.042.67368334