Basic Statistics
Measure | Value |
---|---|
Filename | HH7CGAFX2_n02_ncm10-12.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6758862 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 161928 | 2.395787929979929 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 31106 | 0.46022540480927115 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 7713 | 0.11411684393023559 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 29100 | 0.0 | 47.78018 | 49 |
CGCCGTA | 32655 | 0.0 | 47.132347 | 47 |
TCGCCGT | 33015 | 0.0 | 46.47066 | 46 |
CGTATCA | 29960 | 0.0 | 46.46708 | 50 |
GTATCAT | 29475 | 0.0 | 45.438366 | 51 |
GTGACTG | 3695 | 0.0 | 45.220757 | 1 |
GCCGTAT | 33760 | 0.0 | 45.051167 | 48 |
GTCGCCG | 35005 | 0.0 | 44.788826 | 45 |
CTCGGTG | 31245 | 0.0 | 44.78734 | 38 |
GGTCGCC | 35570 | 0.0 | 44.63832 | 44 |
GATCTCG | 30750 | 0.0 | 44.46109 | 35 |
TCTCGGT | 31135 | 0.0 | 44.383472 | 37 |
ATCTCGG | 31450 | 0.0 | 44.105873 | 36 |
GTGGTCG | 35965 | 0.0 | 43.99234 | 42 |
TGACTGG | 3870 | 0.0 | 43.504112 | 2 |
GGTCGGA | 1605 | 0.0 | 43.398453 | 2 |
GGTGGTC | 36825 | 0.0 | 43.3832 | 41 |
TGGTCGC | 36605 | 0.0 | 43.10843 | 43 |
TCGGTGG | 35550 | 0.0 | 42.858685 | 39 |
AGATCTC | 32120 | 0.0 | 42.673683 | 34 |