FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5516204
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316720.5741629569899881No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC218700.39646829595134625Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA81000.056.7852747
TCGCCGT83500.056.21746
ATCTCGG79850.055.93116436
GGTCGCC99700.055.1928444
CCGTATC76150.054.9337449
GTCGCCG101100.054.70553645
GATCTCG81150.054.34506635
CTCGGTG90800.054.15874538
GCCGTAT89350.053.9863548
TCTCGGT87000.053.26560637
CGTATCA86400.051.05025550
GTGGTCG111150.050.9873942
TGGTCGC100600.050.1060243
AGATCTC83450.048.778434
TCGGTGG126150.048.30472639
GTAGGGA132900.047.89338719
TAGATCT89250.047.49086833
GGTGGTC121800.046.8456941
GTATCAT88750.045.95181751
CGGTGGT126500.045.8741640
Produced by FastQC (version 0.11.5)