FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n02_ncm10-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n02_ncm10-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745575
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1397162.431714841421442Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335690.584258320533628No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC66640.11598491012648864Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA66120.11507986580977536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC242500.048.61964849
CGCCGTA270050.047.7262247
TCGCCGT271650.047.3815146
CGTATCA251050.047.18691350
GTGACTG31250.046.519721
GTATCAT243650.046.378751
GCCGTAT282300.045.049248
GTCGCCG295100.044.8974545
GGTCGCC299900.044.8324944
CTCGGTG276200.044.47982438
GTGGTCG304550.044.1594642
TCTCGGT274350.044.06532337
GACTGGA35950.043.9960063
TGGTCGC303900.043.98898343
ATCTCGG277000.043.79538736
GATCTCG272700.043.6645335
TGACTGG33400.043.385142
GGTGGTC313450.043.2964741
TATCATT269700.042.56101252
AGATCTC281600.042.34665334
Produced by FastQC (version 0.11.5)