FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15395944
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA6511184.229152821028707TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG479620.3115236064771345No Hit
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACA337550.2192460559742228TruSeq Adapter, Index 13 (97% over 35bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC324550.2108022736377841TruSeq Adapter, Index 14 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT287290.1866010944181143TruSeq Adapter, Index 14 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC246590.16016556048787914TruSeq Adapter, Index 14 (97% over 35bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT207820.13498360347374608TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACA206740.13428212001810347TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC199370.1294951449550609Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG190400.12366893514291816TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTG171160.11117213728498883TruSeq Adapter, Index 18 (97% over 42bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT164840.10706716002604323TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG163040.10589802093330557TruSeq Adapter, Index 22 (97% over 49bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC154450.1003186293740741TruSeq Adapter, Index 14 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGC250850.051.2947546
GTGACTG51000.049.0892331
GTGGCGG267900.048.85201348
CGGGGGT274950.047.4830943
GGGTGGA177450.047.48229660
GTGGAAA275050.047.42416862
TGACTGG52250.047.0141642
GGTGGAA182650.046.76250561
TGGCGGG314400.046.28041549
GATCGCG64050.044.55774740
ATCGCGG109600.044.13389241
GGGTGCC77600.043.66176246
GGTGGGA95200.043.0514561
CGCGGGT65150.042.68779843
TCGCGGA39550.042.10024642
GTGCCGG103100.042.03022848
TGCCGGG144200.041.8719949
ATGCCGG82800.041.63951548
GCGGGGT192350.041.56248551
ATCGGGG430850.041.1000641
Produced by FastQC (version 0.11.5)