FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448808
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1520472.7904635289039366TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTGTG98850.18141582525939617TruSeq Adapter, Index 9 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT66420.12189822067505407TruSeq Adapter, Index 9 (100% over 48bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC55190.10128820835676354TruSeq Adapter, Index 9 (100% over 48bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA56650.049.79483860
AGTCGGA17550.047.3080521
GGGTGGA41050.045.3573560
TCGGCGT56150.043.6935657
CGGCGTG59050.041.96261658
GTGGAAA114750.041.44917762
GGGGGTG46600.041.30721758
TGCCGTG62250.041.21106349
GGCGTGG57900.041.1035759
GCCGTGG48150.040.2682450
CGTGGAA72200.039.7974461
TGGGGGT48050.039.6965157
GTCGGCG36150.039.50036256
GCCGTCG100750.038.48964750
CCGTCGT100400.037.9963651
CCGTGGT49400.037.9740651
ATGCCGT301700.037.91018748
TCGTCGG46000.037.6614154
GCGTGAA61900.037.42971860
GTGGGGG53400.037.35793356
Produced by FastQC (version 0.11.5)