FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6887846
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG3606345.235802310330399TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGG1317361.9125863150831188TruSeq Adapter, Index 8 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT296250.43010543499375564TruSeq Adapter, Index 8 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTT262410.3809754166977601TruSeq Adapter, Index 8 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA245020.3557280461845401TruSeq Adapter, Index 8 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG161020.2337741000597284TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG159520.23159635102178536TruSeq Adapter, Index 8 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG122240.1774720282654403TruSeq Adapter, Index 8 (100% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGG96360.13989859819746261TruSeq Adapter, Index 8 (97% over 42bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGG81770.11871635922173637TruSeq Adapter, Index 8 (97% over 42bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT75020.10891648855099256TruSeq Adapter, Index 8 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTG68990.10016193741846145TruSeq Adapter, Index 8 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGCG348750.056.99142547
TGGCGGG367000.056.9610949
AGTCGGA44600.056.702361
GTGGCGG352100.056.52870648
GGGTGGC370650.056.48515346
GGTGGGA88200.051.4663461
CGGGGGT579250.049.64769743
GTCGGAA48300.048.857562
GCGGGGT168300.048.14140351
CGGGGAT31700.046.92254343
GTGGGAA98650.046.90144762
GGGTGGA104950.046.28698360
TGGGAAA105300.044.670763
AAAAAAG774850.043.73651570
TGGGGGT126500.043.6861257
GGTGGAA112500.043.24283261
ATGGCGG26800.042.96481748
AAAAAGG263250.042.51693370
GGATGGC28550.042.41523446
CGGGGTG208850.041.9448152
Produced by FastQC (version 0.11.5)