FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350726
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTG3605714.905243373239595TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGGG964131.3116119414599319TruSeq Adapter, Index 7 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA329850.44873118655218547TruSeq Adapter, Index 7 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTT310450.42233923560747605TruSeq Adapter, Index 7 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC271410.36922883535585466TruSeq Adapter, Index 7 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTG192550.2619469151754534TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTG166760.22686194533710005TruSeq Adapter, Index 7 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGATG157930.21484952642772973TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGGG148750.20236096407348064TruSeq Adapter, Index 7 (97% over 41bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT131580.17900272707757028TruSeq Adapter, Index 7 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGGTG102630.13961886213688282TruSeq Adapter, Index 7 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGTGTG85820.11675037268427636TruSeq Adapter, Index 7 (97% over 42bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA78390.10664252755442115TruSeq Adapter, Index 7 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA44900.058.892821
GGTGGCG390650.054.8747647
GTGGCGG405000.053.58720448
TGGCGGG440400.053.5155749
CGGGGGT543150.053.50839643
GGGTGGC423850.053.40872246
GGTGGGA90600.051.45533461
GTCGGAA49750.049.4705352
GGGTGGA121300.048.0405360
TCGGGGG773950.047.27869442
GTGGGAA101250.046.76889862
ATCGGGG863800.046.4126441
GCGGGGT229200.045.77953351
CGGGGAT33050.045.74742543
CATCGGG906250.045.5902940
GGTGGAA129050.045.34533761
TCATCGG938350.044.0754539
TGGGAAA108750.043.96183463
TCGGGGA35900.043.28554542
AAAAAAG851250.043.08410370
Produced by FastQC (version 0.11.5)