FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6746336
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1495242.2163734507145807TruSeq Adapter, Index 6 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTG1111021.6468494898564199TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTGTG325190.48202461306403954TruSeq Adapter, Index 6 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGGGGGTG240340.35625263846923727TruSeq Adapter, Index 6 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA236210.3501307969244342TruSeq Adapter, Index 6 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTGTG231950.3438162581881483TruSeq Adapter, Index 6 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGGGTG211670.31375549631681554TruSeq Adapter, Index 6 (97% over 45bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT198760.29461918291647493TruSeq Adapter, Index 6 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATG186660.2766835212476817TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGGTG177010.26237946049529703TruSeq Adapter, Index 6 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGGGTGTG166410.24666722795899879TruSeq Adapter, Index 6 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGGGTG110380.1636147384298677TruSeq Adapter, Index 6 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATG101510.15046686082638042TruSeq Adapter, Index 6 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA99780.14790250589356949TruSeq Adapter, Index 6 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTATG89990.13339092508881858TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTG69550.10309299744335296TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA32750.055.943961
GCGTGGA146350.055.50470760
GGGTGGA208900.054.19799860
GTGGAAA369700.053.0797162
GGTGGAA221950.051.5001461
CGTGGAA160100.051.26239461
TGGAAAA431300.048.67146363
CGTGGTG139600.047.8845452
GGTGGGA74450.047.620361
GCCGTGG122400.047.29350350
CCGTGGT125300.046.61789751
GGGCGTG137550.046.1046858
TGGGGGT292650.045.88710457
GTCGGAA37700.045.7887732
TGCCGTG144750.045.62477549
TGGGCGT139500.045.2093257
GTGGGCG135500.043.9093556
TGGTGGG145150.043.66654254
GTGGGGG399350.043.51239456
TGGCGGG178100.043.2517349
Produced by FastQC (version 0.11.5)