FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5755460
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTG2153183.741108443113148TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGGG388080.6742814649046297TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGGTG364080.6325819308969223TruSeq Adapter, Index 5 (97% over 41bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG257960.44820049135950907TruSeq Adapter, Index 5 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGGGGGTG208870.36290756950791075TruSeq Adapter, Index 5 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG198390.3446987729912118TruSeq Adapter, Index 5 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTT151170.2626549398310474TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGATG121640.21134713819573064TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGTGTG120860.20999190334048015TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATG106610.18523280502340386TruSeq Adapter, Index 5 (97% over 41bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT101920.17708402108606436TruSeq Adapter, Index 5 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGGGTG87100.151334558836305TruSeq Adapter, Index 5 (97% over 45bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG65270.11340535769512776TruSeq Adapter, Index 5 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG57850.10051325176441152TruSeq Adapter, Index 5 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA35150.057.4002651
GGGTGGA139900.052.68552460
GATCGGG64750.052.26817340
ATCGGGG55650.051.82182341
GGTGGAA144250.051.77608561
GTGATCG104950.051.12229538
GGTGGGA93650.050.3023161
GGTGGCG326800.050.25961747
GTGGAAA174950.049.97227562
ATCGCGG30150.049.79941
GATCGCG37500.049.65134440
GTGGCGG349450.048.97500248
TGATCGG70750.048.97329739
AGTGATC126750.048.87378337
GGGTGGC352450.048.64750746
GTCGGAA39400.048.2533872
GCGTGGA25700.047.6634660
TGATCGC39850.047.33812739
TGGGAAA108200.046.7402763
GTGGGAA106200.046.5000262
Produced by FastQC (version 0.11.5)