FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6311057
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1092291.730756036587849TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTGTG734131.1632441285192006TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTG180820.28651301992677297TruSeq Adapter, Index 4 (97% over 45bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC144030.22821850602838795TruSeq Adapter, Index 4 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA116480.1845649627312826TruSeq Adapter, Index 4 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGGTG100730.15960876284273776TruSeq Adapter, Index 4 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTGTG88390.14005577829514138TruSeq Adapter, Index 4 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATG73940.11715945522279389TruSeq Adapter, Index 4 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA81800.053.43931260
GGGTGGA99800.048.815860
AGTCGGA25250.047.170291
GTGGAAA202150.046.0012462
GGTGGAA111750.043.8462361
GCCGTGG80250.043.39400550
CGTGGAA101700.043.3267961
CGTGGTG80500.043.25924352
CCGTGGT79050.043.1672651
GGTGGGA47350.042.79670361
TGGGGGT142500.041.92474457
CTCGGGT36400.041.6330543
TGCCGTG95300.041.57241449
GGGCGTG73750.040.95439558
TGGGCGT74000.040.67414557
GTGGGGG193100.040.3273356
TGGAAAA250450.040.12027463
ATCGCGG34200.039.80844541
GGGGGTG161350.039.5429858
GTGGGCG73100.039.45132456
Produced by FastQC (version 0.11.5)