FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358903
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTG1035072.374611226723788TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTG446691.024776187953712TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTT153770.3527722456774101TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGGG142550.3270318242915706TruSeq Adapter, Index 3 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG129240.2964966185299375TruSeq Adapter, Index 3 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC116820.26800321089962315TruSeq Adapter, Index 3 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTT70050.16070557202121727TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGGTG67690.1552913657404168TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTG66150.15175836672667414TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATG58350.13386395613758784TruSeq Adapter, Index 3 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG53850.12354025772080728TruSeq Adapter, Index 3 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT164250.054.7834143
AGTCGGA21050.054.4066431
GGTGGCG160550.053.953247
GTGGCGG166500.052.63472748
TCGGGGG195450.052.26978342
GGGTGGC183100.051.2652146
ATCGGGG228650.050.5732841
TGGCGGG190100.050.2427949
CATCGGG247350.048.9854940
GGTGGGA42750.048.38421261
GCATCGG258150.047.50554339
GGGTGGA65350.047.39690860
GCGGGGT120150.046.98539751
GTCGGAA23850.045.948122
GGCATCG295050.045.83463738
AGGCATC319250.044.76110537
CGGGGTG141950.044.60211652
GGTGGAA72150.043.31790561
GTGGGAA52050.041.4874562
GGCGGGG250000.040.93451750
Produced by FastQC (version 0.11.5)