FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429586
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTG2200063.4217755233385168TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGGG384860.5985766424152348TruSeq Adapter, Index 2 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT171730.26709340228126666TruSeq Adapter, Index 2 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTT155620.24203735668206322TruSeq Adapter, Index 2 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT147790.22985927865340006TruSeq Adapter, Index 2 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATG146760.22825730925754786TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTGTG141040.21936093552524222TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGGTG96140.14952751234682918TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTG94720.14731897201468336TruSeq Adapter, Index 2 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG74840.11639940736464215TruSeq Adapter, Index 2 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA27300.054.020741
GGTGGGA78650.050.6843861
GGTGGCG276650.048.75623747
GGGTGGC298250.047.5603846
TGGCGGG313000.047.2982649
GTGGCGG299100.046.9103648
GGGTGGA109550.046.00438760
GTGGGAA91400.044.95427762
GTCGGAA31850.044.6328772
CGGGGGT401450.043.694743
TGGGGGT155500.043.1010257
TGGGAAA98300.042.3684563
GGTGGAA121500.042.08458761
GCGGGGT202400.041.94975351
ATGGCGG30300.040.5427948
GTGGAAA146250.040.3230362
ATGCCGG19000.040.15614748
GTGCCGG44800.039.99825748
CGGGGAT33700.039.67186443
GGATGGC28600.039.5262546
Produced by FastQC (version 0.11.5)