FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6278580
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGGGGGTG1728162.7524695074363947TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGGGGGGG358500.5709889815850081TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGGGTG217400.3462566376473661TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGGGGGTT216290.3444887219721657TruSeq Adapter, Index 22 (97% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC136470.21735806504018426TruSeq Adapter, Index 22 (97% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTA135730.2161794545900506TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGGGGTTG106930.17030921004430938TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGGGTGTG95290.1517699862070723TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTGGGGTG82440.1313035750121843TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA29300.052.366191
GGTGGCG221750.051.8783547
GTGGCGG248050.049.9898748
TGGCGGG281550.048.38016549
GGGTGGC257100.048.31188246
CGGGGGT303350.048.05306243
GGTGGGA64450.047.0810661
TCGGGGG391100.044.5110742
GGGTGGA108650.044.45282760
GCGGGGT185150.044.2325751
GTCGGAA32450.043.8060762
ATCGGGG443300.043.31988541
CGGGGTG225650.041.5205552
CATCGGG494700.041.5072840
GTGGGAA75600.041.2482962
GGTGGAA119950.040.7611361
AAAAAAG529100.040.21757570
GCATCGG516850.040.07379539
GTGGAAA142800.039.2385762
GGCGGGG372450.039.1660550
Produced by FastQC (version 0.11.5)