FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5850817
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGGGGGTG1731912.9601165102241276TruSeq Adapter, Index 7 (97% over 36bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT134930.23061736506200758TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGGGGGTT133020.22735286371117058TruSeq Adapter, Index 7 (97% over 36bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG126620.21641422044135034TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGGGTGTG84960.14521048940686404TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGGGTG80390.137399614447008TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGGGGATG73430.12550383989107847TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA24800.050.28341
GGTGGGA64150.048.06517461
GGTGGCG221150.045.8155547
GGGTGGC241250.044.0874246
TGGCGGG243100.043.9678649
GTGGCGG239550.043.91815648
CGGGGGT315650.043.73029343
GTGGGAA75250.042.8820762
GGGTGCC41050.040.9241246
GTCGGAA29250.040.3380552
TCGGGGG416950.039.93010742
GGGTGGA77100.039.90118460
GCGGGGT152600.039.70030651
TGGGAAA82700.039.6538563
GTGCCGG44000.039.13485348
AAAAAAG441900.038.83986770
ATCGGGG469550.038.4161541
CGGGGTG187100.038.1412352
TGGGGGT118350.037.73407457
GATCGGG516950.036.70813440
Produced by FastQC (version 0.11.5)