FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5524013
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1746113.1609447696810276TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTGTG343420.6216857201458432TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA193990.3511758571169184TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA135420.2451478662342033TruSeq Adapter, Index 1 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG117810.21326886812178028TruSeq Adapter, Index 1 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATG77290.13991639773476275TruSeq Adapter, Index 1 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT68520.12404025841358447TruSeq Adapter, Index 1 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATG61560.11144072253269498TruSeq Adapter, Index 1 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC56290.10190055671483757TruSeq Adapter, Index 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA86000.051.80607660
AGTCGGA22850.049.6678431
GGGTGGA79300.048.41547460
GTGGAAA178050.046.7041262
CGTGGAA99950.044.9606961
GGTGGAA88300.043.59962561
CGTGGTG86500.042.32201452
GCCGTGG97250.042.25024450
CCGTGGT97000.041.8179251
TGGAAAA231200.041.7954863
TGGGGGT108100.041.3119557
TGCCGTG121000.040.95704349
GGGGGTG110650.040.0435958
TGGTGGG88200.038.8476654
TCGGCGT78150.038.8276357
GGCGTGG108000.038.6280959
GTCGGAA26750.038.611622
GTGGGGG130450.038.23151456
GGAAAAA286500.038.18696264
GGGCGTG73550.038.11540658
Produced by FastQC (version 0.11.5)