FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102188
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG4458517.3064120607231375TruSeq Adapter, Index 22 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTGTG850191.3932543540120363TruSeq Adapter, Index 22 (97% over 46bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA306250.5018691656173163TruSeq Adapter, Index 22 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG253900.4160802649803644TruSeq Adapter, Index 22 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATG194050.31800069089972316TruSeq Adapter, Index 22 (97% over 42bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT124890.2046642941843155TruSeq Adapter, Index 22 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTGTG115050.18853893062619506TruSeq Adapter, Index 22 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAC113360.18576943221021705TruSeq Adapter, Index 22 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGATG113340.1857366570810339TruSeq Adapter, Index 22 (97% over 45bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC110630.18129562707671412TruSeq Adapter, Index 22 (97% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT103980.17039789662330954TruSeq Adapter, Index 22 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGGTG92030.15081475693636445TruSeq Adapter, Index 22 (97% over 45bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA85310.1398023135308188TruSeq Adapter, Index 22 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC71910.11784297697809375TruSeq Adapter, Index 22 (98% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT69770.11433603815549438TruSeq Adapter, Index 22 (97% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61060.10006246939622312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA38100.057.272911
GCGTGGA207550.056.2541960
GTGGAAA379350.054.22095562
CGTGGAA218500.053.70734461
GGGTGGA166000.052.89859860
TGGAAAA465400.051.19702563
GGTGGAA173350.051.0998861
GTCGGAA42750.047.6652832
GCCGTGG206800.047.2008250
GGTGGGA50950.046.91676761
CGTGGTG180150.046.89802652
CCGTGGT205250.045.98851451
TGGGGGT213450.045.9598457
TGGTGGG173900.045.5043154
TGCCGTG260150.045.28369549
GGGGGTG221400.045.05249458
TCGCGGG15450.043.94655642
GGAAAAA613750.043.868864
GGCGTGG240650.043.3829459
ATCGCGG26000.043.3445141
Produced by FastQC (version 0.11.5)